Fig. 4.

Gene Expression levels of humoral factors in in vitro-transcribed mRNA (IVT mRNA)-transfected fibroblast-like synoviocytes (FLS) and MH7A cells. RT-qPCR of IVT mRNA-transfected cells showed changes in the expression of three candidate genes. a–c FLS (n = 3): a IL6, b TNF, and c CXCL10. Gene expression levels were normalized to those of GAPDH. d–f MH7A (n = 4): d IL6, e TNF, and f CXCL10. Gene expression levels were normalized to those of GAPDH. The samples were harvested at 24 h after transfection. Data are presented as mean ± SEM. t-test, *p < 0.05, **p < 0.01