Figure 10.

Fluoro-Jade C staining was performed to confirm that the impact procedure did not induce immediate cell death. Using the brains of the animals sacrificed at 1-day post-impact (Cohort 1), all brain regions were examined to ensure accuracy. (A) Representative image of the sham cortex with no visible cell death. (B) Representative image of the cortex (location of impact) of a 2 hits/4 h animal with no visible cell death. (C) Representative image of the cortex of a 4 hits/4 h animal with no visible cell death. (D) Representative image of the cortex of an 8 hits/4 h animal with no visible cell death. (E) A positive control, a controlled cortical impact brain, where there is known cell death, was included for reference. The controlled cortical impact brain shows definite positive staining. (F) Overlay of Fluoro-Jade and DAPI staining of the controlled cortical impact brain to confirm that the positive signal was, in fact, representative of cell death.