Figure 2.

The experimental outline includes three cohorts of animals. Each cohort was comprised of 4 groups, sham, 2 hits in 4 h, 4 hits in 4 h, and 8 hits in 4 h. (A) For each of these groups, both the number of impacts sustained, as well as interval between impacts, differed. For the 2 hits/4 h group, there was a 2-h interval between impacts. For the 4 hits/4 h group, there was a 1-h interval between impacts. For the 8 hits/4 h group, there was a 30-min interval between impacts. Shams were split across each of these groups, so that some sham animals received isoflurane exposures alongside the 2 hit group, others alongside the 4 hits group or the 8 hits group. (B) Cohort 1 received locomotor testing at 1-day post-impact and were sacrificed for histological outcomes immediately following. Cohort 2 received locomotor testing at 1 week and were sacrificed for histological outcomes at that time as well. Cohort 3 received locomotor testing at 1-day post-impact, 1-week post-impact, and 1-month post-impact. Cohort 3 also underwent “full behavioral testing” starting at 2-weeks post-impact including Rotarod, Open Field Test, Elevated Plus Maze, Morris Water Maze, and the Von Frey test. Cohort 3 was sacrificed for histological outcomes at 1-month post-impact.