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. 2022 Jun 16;13:857654. doi: 10.3389/fneur.2022.857654

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Copyright © 2022 Boucher, Conley, Nowlin, Qiu, Kawata, Bazarian, Meehan and Mannix.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Locomotor activity was measured for each cohort of animals as the total distance traveled (cm) over a 10-min period. (A) Cohort 1 underwent locomotor testing at 1-day post-impact. There was a main effect of impact condition for Cohort 1 (F(3, 20) = 7.04, p = 0.0020**), with post-hoc tests revealing that the 2 hits group (5432.17 ± 200.13) had significantly higher locomotor activity than the 4 hits (4539.33 ± 199.16; p = 0.017*) and 8 hits animals (4377.50 ± 135.22; p = 0.0014**) (sham: 48.36 ± 127.41; other ps > 0.09). (B) Cohort 2 underwent locomotor testing at 1-week post-impact. There was no main effect of impact condition for Cohort 2 (F(3, 20) = 0.77, p = 0.53).