FIGURE 10.

Protein expression levels in urothelium homogenates prepared from detrusor-free bladder preparations. Representative immunoelectropherograms (duplicates) of nucleotidases detected in urothelium using ProteinSimple Wes (A–J). Each antibody was diluted 100-fold and each well contained 6 µg of urothelium homogenate sample. The antibodies used are described in Figure 9 and in main text Antibodies. (K) Scatter plots of AUC of chemiluminescence (CL) signals normalized per µg loaded urothelium sample. Each symbol represents a single loading from 5 urothelium samples, loaded in triplicates. Statistical significance is described in main text Results.