FIGURE 11.

Protein expression levels in cELS collected from distended detrusor-free bladder preparations. Representative immunoelectropherograms (duplicates) of nucleotidases detected in cELS using ProteinSimple Wes (A–J). Each antibody was diluted 100-fold and each well contained 3 µl of cELS sample. The antibodies used are described in Figure 9 and in main text Antibodies. (K) Scatter plots of AUC of chemiluminescence (CL) signals normalized per µL loaded cELS sample. Each symbol represents a single loading from 3 cELS samples, loaded in triplicates. Statistical significance is described in main text Results.