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. 2022 Jun 16;13:918100. doi: 10.3389/fphys.2022.918100

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Copyright © 2022 Aresta Branco, Gutierrez Cruz, Dayton, Perrino and Mutafova-Yambolieva.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Degradation of eATP and eADP by soluble enzymes released in the LP at rest and during bladder filling. (A) Summarized data showing the degradation of eATP in diluted (i.e., 2.5-ml) EL samples collected at different time points during 1 h after addition of eATP in the absence of bladder preparation (Protocol 2). eATP, eADP, eAMP, and eADO are presented as percentages of total purines (eATP + eADP + eAMP + eADO) present in EL solution samples from nondistended (dotted connecting lines) and distended (solid connecting lines) preparations. (B) Summarized data showing the degradation of eADP in diluted (i.e., 2.5-ml) EL samples collected at different time points during 1 h after addition of eADP in the absence of bladder preparation (Protocol 2). eADP, eAMP, and eADO are presented as percentages of total purines (eADP + eAMP + eADO) present in EL solution samples from nondistended (dotted connecting lines) and distended (solid connecting lines) preparations. (C) Original chromatograms of eATP in beaker (blue) and at 60 min of addition of eATP to concentrated EL samples (Protocol 3) collected from nondistended (red) and distended (green) bladder preparations. The chromatograms demonstrate greater decrease of eATP and increase in eADP, eAMP, and eADO in EL solutions from distended than from nondistended preparations. (D) Original chromatograms of eADP in beaker (blue) and at 60 min of addition of eADP to concentrated EL samples (Protocol 3) collected from nondistended (red) and distended (green) bladder preparations. The chromatograms demonstrate greater decrease of eADP and increase in eAMP and eADO in EL solutions from distended than from nondistended preparations. (E) Summarized data showing the degradation of eATP in EL samples collected at different time points during 1 h after addition of eATP in concentrated EL samples collected from nondistended (dotted connecting lines) and distended (solid connecting lines) (Protocol 3). eATP, eADP, eAMP, and eADO are presented as percentages of total purines (eATP + eADP + eAMP + eADO) present in the EL solution samples. (F) Summarized data showing the degradation of eADP in EL samples collected at different time points during 1 h after addition of eADP in concentrated EL samples collected from nondistended (dotted connecting lines) and distended (solid connecting lines) preparations (Protocol 3). eADP, eAMP, and eADO are presented as percentages of total purines (eADP + eAMP + eADO) present in the EL solution samples. Statistical significance is described in main text Results.