Fig 1. Generation and characterization of a transgenic mouse line to study endogenous Pants.
A) CRISPR/Cas9 strategy to target the miniSOG and HA tags to the C-terminus of the endogenous Pants locus. B) Genotyping verification of insert in mouse tail biopsies. C) Quantitative Western blot of Pants immunoprecipitated from hippocampus of PantsSOG-Y and WT control mice at 8 and 16 weeks immunoblotted with HA antibody. D) Quantification of HA bands from (c), normalized to β-actin controls (n = 3, P = 0.004). E) Immunohistochemistry with HA antibody shows Pants localization in the hippocampus at 8 and 16 weeks.