Skip to main content
. 2022 Jun 30;17(6):e0269404. doi: 10.1371/journal.pone.0269404

Fig 3. Extrasynaptic Pants is downregulated in response to neuronal activity.

Fig 3

A) Contrasting extracellular/intracellular immunocytochemistry staining for HA in cultured hippocampal neurons. B-C) Co-localization of intra- and extra-cellular Pants with synaptic markers D) Quantification of Pants co-localization with pre- and post-synaptic markers correlation coefficients. E) Differentiated neuronal extracellular and internalized HA-Pants staining with and without 10 seconds 60mM KCl stimulation prior to fixation. Graphs at the right show quantification of HA fluorescence in dendritic ROIs, normalized to MAP2/Shank2 fluorescence. n = 3 P<0.001(extracellular) and P = 0.005 (internalized)P values generated by Mann-Whitney Rank Sum test.