TABLE 5.

PrtH activity of L. helveticus CRL 1062 after growth in SCDM supplemented with different peptides^a

Addition to basal SCDM	PrtH sp act (nmol min−1 mg of protein−1)b
None	192 ± 8
1% LMMP	18 ± 1
1% HMMP	191 ± 8
1 mM Leu-Gly-Glyc	190 ± 7
1 mM Leu-Leud	189 ± 8
5 mM Leu-Leud	185 ± 9
1 mM Leu-Pro	191 ± 7
5 mM Leu-Pro	144 ± 5

^aValues are the means and standard deviations from three independent experiments. 

^bLDH activity was less than 0.06% the total activity in each cell extract. 

^cThe PrtH activity of L. helveticus CRL 1062 after growth in SCDM with the tripeptide Val-Pro-Leu was similar. 

^dThe PrtH activity of L. helveticus CRL 1062 after growth in SCDM with the dipeptides Gly-Met, Gly-Pro, Gly-Tyr, Pro-Ala, Pro-Leu, and Tyr-Gly was similar.