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. 2022 Jun 16;9:893977. doi: 10.3389/fsurg.2022.893977

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Copyright © 2022 Zhou, Yang, Li, Xiong, Zhang and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression of NCBP2 promotes the proliferation of LC cells. (A) RT-qPCR detection on 15 pairs of clinical samples confirmed the differential mRNA expressions of NCBP2 between adjacent normal and HCC tissues. (B) Western blot results for four pairs of clinical samples confirming the differential protein expressions of NCBP2. (C) Expressive differences of NCBP2 between normal liver and LC cells. (D,E) Transfection efficiency of si-NCBP2 and OE-NCBP2 in HepG2 and HuH-7 cells based on RT-qPCR tests. (F,G) Effects of si-NCBP2 and OE-NCBP2 on protein expression of NCBP2 in LC cells based on western blot assays. (H,I) CCK8 assays exhibited the proliferative abilities of LC cells in different groups. LC, liver cancer; si-NCBP2, small interfering RNA targeting NCBP2; OE, overexpression; *P < 0.05, **P < 0.01, ***P < 0.001.