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. 2022 Jun 28;11:e72083. doi: 10.7554/eLife.72083

Figure 4. Comparison of nested PCR (nPCR) and droplet digital PCR (ddPCR) for hrp2 deletion typing.

Figure 4.

Representative examples of results obtained by hrp2 and msp2 nPCR, and by ddPCR. The expected size of the hrp2 band is 228 bp. A band is visible in samples 3, 4, and 5, but no band is visible in samples 1, 2, 6, and 7. msp2 was run as control for the nPCR assay. msp2 is a size polymorphic gene with amplicons ranging from approximately 200–500 bp. Bands are observed for all samples, and multiple bands are observed in case of polyclonal infections. L = 100 bp DNA ladder (New England BioLabs). Samples were run in triplicate, and the same results were obtained all three times. By ddPCR, no deletions were observed in any samples. For samples 1 and 7, the hrp2 exon 2/tRNA ddPCR plot is shown. Droplets are visible for both targets, thus no deletion is observed.