Table 1. Primer and probe sequences.
Assay | Sequence 5′–3′ | |
---|---|---|
hrp2 exon 2 forward | CATTTTTAAATGCTTTTTTATTTTTATATAG | |
hrp2 exon 2 | hrp2 exon 2 reverse | CTTGAGTTTCGTGTAATAATCTC |
hrp2 exon 2 probe | FAM-CGCATTTAATAATAACTTGTGTAGCAAAAATGC-BHQ-1 | |
hrp2 exon 1 forward | ATATTTATACATTTTTGTTATTATTTCTTTTTC | |
hrp2 exon 1 | hrp2 exon 1 reverse | CGTTATCTAACAAAAGTACGGAG |
hrp2 exon 1 probe | FAM-CAAAAACGGCAGCGGATAATACTT-BHQ-1 | |
hrp3 forward | ATGCTAATCACGGATTTCATTTTA | |
hrp3 | hrp3 reverse | ATCGTCATGGTGAGAATCATC |
hrp3 probe | FAM-CCTTCACGATAACAATTCCCATACTTTAC-BHQ-1 | |
tRNA forward | CATCAAATGAAGATTTAACAAGAG | |
tRNA | tRNA reverse | CTTTTTGATTCTATAGTTTCATCTTTATG |
tRNA probe | HEX-CTACCTCAGAACAACCATTATGTGCT-BHQ-1 |