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. 2022 Jun 20;11:e76923. doi: 10.7554/eLife.76923

Figure 5. Myo-inositol suppresses aggregation more strongly in redox buffers.

Figure 5.

(A, B) Turbidity traces of 50 μM W42Q in buffers with set redox potentials using the glutathione redox couple, where OxD = 2[GSSG]/([GSH]+2[GSSG]), and ([GSH]+2[GSSG])=2 mM in all cases. The OxD range shown is consistent with what is observed during cataract development (Hains and Truscott, 2007). Notably, in the least-oxidizing buffers with myo-inositol, the turbidity curves became biphasic. (C) Quantification of the maximum aggregation rates and lag times for the curves in (A) and (B) showed stronger aggregation suppression by 100 mM myo-inositol than in Figure 1A and B. (D) Ratios of maximum aggregation rates (+inositol/-inositol) (top) and aggregation lag times (bottom), for the data shown in panel (C). Averaged across the six OxD buffers, mean ± S.E.M. for inositol’s inhibitory effect on the aggregation rate was 51±2% vs 35 ± 3% observed in the fully oxidizing buffer.