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. 2022 Jul 1;132(13):e158885. doi: 10.1172/JCI158885

Figure 1. Follicular B cells adoptively transferred into lymphopenic hosts progressively acquire a marginal zone B cell phenotype.

Figure 1

Highly purified congenically marked B6-CD45.1+ FoB cells were adoptively transferred (i.v.) into B6 or Rag2–/– hosts. Flow cytometry was performed on recipient spleen on days 2, 4, and 8 after transfer. (A) Experimental model. (B) Flow cytometric identification of donor-derived CD45.1+ cells recovered from B6 and Rag2–/– mice 8 days after transfer, gated on single live lymphocytes. (C) Absolute number of CD45.1+ donor-derived lymphocytes at days 2, 4, and 8 after transfer in B6 (black) or Rag2–/– (red) recipients. Data shown as mean ± SEM. (D) Analysis of adoptively transferred CD45.1+ FoB cells 2, 4, and 8 days after transfer by CD23 and CD1d expression in B6 (left) and Rag2–/– (right) recipients. Arrows depict shifts in cell surface phenotype. (E) Percentage of CD45.1+ cells that acquired a MZB cell phenotype by days 2, 4, and 8. Each data point represents an individual mouse. *P < 0.05, ***P < 0.001, and ****P < 0.0001, by 2-way ANOVA.