Figure 2. Follicular B cells transferred into lymphopenic hosts acquire a marginal zone B cell phenotype and then proliferate.

Highly purified congenically marked B6-CD45.1⁺ FoB cells were labeled with CTV and adoptively transferred (i.v.) into B6 or Rag2^–/– hosts. Flow cytometry was performed on recipient spleen on days 2, 4, and 8 after transfer. (A) Histograms depicting cell surface expression of markers and CTV dilution in CD45.1⁺ B cells recovered from B6 (black) and Rag2^–/– (red) recipients. One representative sample of 3–4 mice is shown. (B) Quantification of the MFI for the indicated markers. Each data point represents an individual mouse. *P < 0.05, ***P < 0.001, and ****P < 0.0001, by 2-way ANOVA.