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. 2022 Jul 1;132(13):e158885. doi: 10.1172/JCI158885

Figure 6. Notch2 signals are required for the transdifferentiation of follicular B cells into marginal zone–like B cells in lymphopenic recipients.

Figure 6

Congenically marked CD45.1+ FoB cells were labeled with CTV and adoptively transferred into B6 or Rag2–/– hosts treated with isotype control or anti-Notch2 antibodies on days 0, 3, and 6 after transfer. (A) Representative flow cytometry plots depicting live CD45.1+CD19+CD93– donor-derived mature B cells recovered at day 8 from B6 or indicated Rag2–/– recipients. (B) Percentage of CD45.1+ MZB cells at day 8 in each recipient group. Data shown as mean ± SEM. (C) Histograms depicting cell surface expression of markers and CTV dilution among donor-derived CD45.1+ B cells in B6 (black), Rag2–/– recipients treated with isotype control antibodies (Iso, red), or Rag2–/– recipients treated with anti-Notch2 antibodies (green). Results are representative of 2 independent experiments. (D) Quantification of the MFI for the indicated markers. (B and D) *P < 0.05, ***P < 0.001, and ****P < 0.0001, by 1-way ANOVA. Each data point represents an individual mouse.