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. 2022 Jul 1;132(13):e158885. doi: 10.1172/JCI158885

Figure 7. B cell homeostatic responses to lymphopenia depends on Delta-like 1 Notch ligands.

Figure 7

Congenically marked CD45.2+ FoB cells were labeled with eF450 and adoptively transferred into B6-CD45.1 or Rag2–/– recipients treated with isotype control or anti-Dll1, anti-Dll4, or both antibodies at days 0, 3, and 6 after transfer. Flow cytometric analysis of recipient spleens was performed at day 8 after transfer. (A) Experimental model. (B) Representative flow cytometry plots gated on CD45.2+CD19+CD93– live donor-derived B cells recovered at day 8 from B6-CD45.1 mice or CD19+CD93– B cells from indicated Rag2–/– recipient groups. (C) Percentage of donor-derived marginal zone–like B cells at day 8 in each recipient group. Each data point represents an individual mouse. Data shown as mean ± SEM. (D) Histograms depicting cell surface expression of the indicated markers and eF450 dilution among donor-derived B cells from indicated B6-CD45.1 or Rag2–/– recipients. **P < 0.01 and ****P < 0.0001, by 1-way ANOVA.