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. 2022 Jul 1;132(13):e150951. doi: 10.1172/JCI150951

Figure 6. Denatonium-induced neurogenic inflammation is CGRP and SP dependent.

Figure 6

(A) Quantification of Evans blue (EB) intensity in response to 1, 10, or 20 mM denatonium in Trpm5+/+ mice after treatment with the CGRP receptor antagonist CGRP8–37. (B) Analysis of neutrophils per tracheal ring in Trpm5+/+ mice treated with CGRP8–37. (C) Evaluation of EB intensity in response to 1, 10, or 20 mM denatonium in Trpm5+/+ mice treated with the NK1-R inhibitor CP96345. (D) Analysis of neutrophils per tracheal ring in Trpm5+/+ mice treated with CP96345. (E) Evaluation of EB intensity in SP–/– and WT mice (SP+/+) stimulated with vehicle or denatonium (20 mM) revealed reduced EB extravasation in SP–/– mice in response to denatonium. (F) Analysis of neutrophils per tracheal ring in SP–/– mice. In A–F, n = 14–20 tracheal rings of 3–4 mice. Data are shown as single values ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 by 1-way ANOVA followed by Bonferroni’s multiple-comparison correction.