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. 2022 Jul 1;132(13):e145099. doi: 10.1172/JCI145099

Figure 2. RBM10 modulates the apoptotic response to osimertinib in EGFR-mutant LA.

Figure 2

(A–D) H3255 and PC-9 (mutant EGFR and WT RBM10) cells expressing shRBM10 or the shScr control were treated with the third-generation EGFR inhibitor osimertinib (500 nM) or DMSO for 48–72 hours. Western blot analysis of the indicated proteins from cellular protein extracts was normalized to actin. (A and B) Quantification of cleaved PARP was determined by signal densitometry. (C and D) The apoptotic response was assessed using a Caspase-Glo 3/7 assay. Each bar represents the mean ± SEM of the FC after normalization to the DMSO control. (E and F) RBM10-deficient A014 (EGFR-mutant and RBM10 Q255*) cells with genetic reconstitution of WT RBM10 were treated with osimertinib (500 nM) for 48 hours. Western blotting of the indicated lysates was normalized to actin (E). Caspase 3/-7 activity was measured using a Caspase-Glo 3/7 assay. Each bar represents the mean ± SEM of the FC after normalization to DMSO control. (F). Data represent 3 independent experiments. *P < 0.05, by 1-way ANOVA. Osi, osimetertinib.