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. 2022 Apr 12;41(3):137–144. doi: 10.12938/bmfh.2021-072

Fig. 2.

Fig. 2.

The hot water extract of G. hansenii GK-1 (FII) inhibited the IL-4 production of spleen cells, but not that of ovalbumin (OVA)-specific CD4+T cells, purified from food-allergic model mice. (A, B) Spleen cells of OVA23-3 mice or (C, D) spleen CD4+T cells of R23-3 mice and antigen-presenting cells prepared from spleens of BALB/c mice were stimulated with G. hansenii GK-1 fractions (FI, FII, and FIII; final concentrations 0.05, 0.2, 1, and 5 µg/mL) and OVA (final concentration 1 mg/mL). The cells were cultured for 24 hr, and (A, C) IL-4 and (B, D) IFN-γ production in the collected culture supernatant was measured by ELISA. The cells of 4 mice were pooled, cultured and measured for 3 wells under each condition. Significance was determined by Dunnett’s test for each G. hansenii GK-1 fraction vs. without each G. hansenii GK-1 fraction (*p<0.05, **p<0.01, #p<0.1). The data are representative of two independent experiments.