TABLE 1.
Characteristics of bacterial strain and plasmids used in this study
| Designation | Relevant characteristics | Source or reference |
|---|---|---|
| Escherichia coli | ||
| DH5α | supE44 ΔlacU169 (f80 lacZΔM15) hsdR16 recA1 endA1 gyrA96 thi-1 relA1, Nalr | Life Technologies |
| LS6164 (or D10) | fadR ΔfadL | 14 |
| LS6164Km2 | Tn5tac1 insertion in LS6164, Kmr | This study |
| 34 | ||
| Sm10λpir | thi-1 thr leu tonA lacY supE recA::RP4-2-Tc::Mu, Kmr λpir | |
| Enterobacter cloacae | ||
| EcCT-501R3 | Wild type, spontaneous Rifr | 43 |
| Ec31 | fadB::Tn5phoA, Kmr | This study |
| EcL1 | ΔfadL::nptII | This study |
| Plasmids and phage | ||
| pWSK29 | Low-copy-number cloning vector, Ampr | 55 |
| pBCKS− | Cloning vector, Cmr | Stratagene |
| pUC19 | Cloning vector, Ampr | 58 |
| pBS II SK+ | Cloning vector, Ampr | Stratagene |
| PUTKm | Tn5-based delivery plasmid, Kmr Ampr | 20 |
| pN103 (or pACC) | Subclone containing E. coli fadL in pACYC177, Ampr | 2 |
| pRK2073 | A pRK2013 derivative with a TnZ insertion in Kmr gene: mobilizing | 11, 28 |
| pCPP2277 | Promoterless uidA adjacent to nptII lacking a transcriptional terminator in pBS II SK− | D. Bauer, Cornell |
| pKV311 | PCR clone of E. cloacae fadBA in pWSK29, Ampr | This study |
| pKVL1 | fadL on ∼30-kb fragment of EcCT-501R3 genomic DNA on pLAFR3 | This study |
| pKVL16 | PCR clone of E. cloacae fadL in pWSK29, Ampr | This study |
| λ::Tn5tac1 | Tn5 with outwardfacing Ptac | 4 |