TABLE 1.
Stepb | Total vol (ml) | Total protein (mg) | Total activity (107 aU) | Sp act (102 aU/mg) | Purification (fold) | Yield (%) |
---|---|---|---|---|---|---|
Culture broth | 10,000 | 160,000 | 1.6 | 1.0 | 1.0 | 100 |
Ultrafiltration | 880 | 44,000 | 1.1 | 2.5 | 2.5 | 69 |
QAE-Sephadex | 1,000 | 9,200 | 1.0 | 11 | 11 | 63 |
Butyl-Toyopearl | 2,400 | 31 | 0.6 | 1,900 | 1,900 | 38 |
Fractogel EMD COO− | 44 | 1.5 | 0.2 | 13,000 | 13,000 | 13 |
Purification procedures are described in Materials and Methods; 1 aU, the amount of killer protein that caused a clear zone of 1 mm.
QAE, quaternary aminoethyl.