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. 2022 Jun 20;7(7):986–1000. doi: 10.1038/s41564-022-01152-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — TLC of lipid standards and Folch (non-acidic) lipid extractions from iSPT and ΔBT_1524 strains of BT. Lanes 1–8, left to right: LPC = 16:0 lyso-phosphatidylcholine; PI-DAG = 16:0 phosphatidylinositol; PC = 16:0 phosphatidylcholine; CPE = ceramide phosphoethanolamine; PE = egg yolk phosphatidylethanolamine; CL = cardiolipin (from bovine heart); Sa = d18:0 sphinganine; Cer = d18:1/18:0 ceramide. Following the dashed white line, in lanes 9–10, are iSPT BT lipid extracts from cells grown with 0 or 100 ng/mL aTC induction of SPT. In the final two lanes (11–12) are lipid extracts from ΔBT_1524 BT. “A” and “B” refer to independently generated knockout strains, both confirmed by Sanger sequencing. The retention factors for inositol lipids (phosphatidylinositol “PI-DAG” and phosphoinositol dihydroceramide “PI-DHC”) are indicated by labels to the right of the figure.

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