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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Anal Bioanal Chem. 2022 Feb 9;414(18):5461–5472. doi: 10.1007/s00216-022-03934-7

Figure 1.

Figure 1.

General workflow utilized throughout the experiment. Samples were run sequentially at different temperatures (Run 1: 30°C, Run 2: 45°C, Run 3: 60°C). Data was collected on a Thermo Q-Exactive-HF orbitrap mass sepctrometer, with peptide and glycopeptide annotations provided by PEAKS and Byonic prior to custom data analysis.