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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Anal Bioanal Chem. 2022 Feb 9;414(18):5461–5472. doi: 10.1007/s00216-022-03934-7

Figure 6.

Figure 6.

Extract ion chromatogram (EIC) of a representative glycopeptide (QN*GTLSK + HexNAc(4)Hex(5)NeuAc(1)), which was identified on all columns at all temperatures. As the retention mechanism of RPLC is driven by hydrophobic interactions, the lack of any liquid-phase resolution is expected. However, both a longer (L=30cm) and short (L=15cm) PGC stationary phases were shown to provide adequate liquid phase resolution of isomeric glycopeptides. Note, the 3+ charge state in BP, 30°C has been manually confirmed as correct.