Table 1.
Parameter | 28 days treatment (mg/kg/day) | 14 days recovery (mg/kg/day) | ||||
---|---|---|---|---|---|---|
G1 (0) | G2 (100) | G3 (300) | G4 (1000) | G1R (0) | G4R (1000) | |
Males | ||||||
RBC (106/µL) | 7.60 ± 0.05 | 7.74 ± 0.12 | 6.50 ± 1.64 | 7.67 ± 0.24 | 8.18 ± 0.41 | 6.73 ± 1.15# |
HGB (g/dL) | 13.40 ± 0.00 | 13.42 ± 0.22 | 12.60 ± 2.64 | 13.64 ± 0.48 | 13.96 ± 0.64 | 11.90 ± 2.14 |
HCT (%) | 41.38 ± 0.31 | 42.04 ± 0.28 | 38.30 ± 6.82 | 42.08 ± 1.87 | 43.48 ± 2.35 | 39.42 ± 2.18# |
MCV (fL) | 54.46 ± 0.09 | 54.34 ± 0.62 | 60.00 ± 5.41* | 54.84 ± 0.71 | 53.14 ± 0.92 | 59.94 ± 11.08 |
MCH (pg) | 17.64 ± 0.13 | 17.36 ± 0.53 | 19.62 ± 1.42 | 17.78 ± 0.13 | 17.08 ± 0.54 | 17.66 ± 0.55 |
MCHC (g/dL) | 32.36 ± 0.26 | 31.92 ± 0.63 | 32.78 ± 1.39 | 32.42 ± 0.39 | 32.12 ± 0.82 | 30.20 ± 5.09 |
PLT (103/µL) | 747.00 ± 15.92 | 762.80 ± 151.91 | 534.60 ± 112.18* | 696.60 ± 35.18 | 851.40 ± 212.34 | 833.20 ± 78.71 |
RET (%) | 1.12 ± 0.23 | 1.08 ± 0.23 | 1.08 ± 0.30 | 1.00 ± 0.20 | 1.08 ± 0.23 | 1.04 ± 0.26 |
WBC (103/µL) | 13.38 ± 0.16 | 12.36 ± 0.21 | 17.52 ± 2.44* | 13.00 ± 1.12 | 18.00 ± 3.57 | 16.50 ± 4.37 |
NEU (%) | 25.80 ± 2.39 | 26.40 ± 2.51 | 26.00 ± 1.87 | 26.00 ± 1.58 | 27.20 ± 1.92 | 26.60 ± 2.07 |
LYM (%) | 72.20 ± 2.28 | 71.80 ± 1.92 | 72.40 ± 2.07 | 72.20 ± 2.28 | 71.00 ± 1.58 | 71.60 ± 1.95 |
MONO (%) | 1.20 ± 0.84 | 0.60 ± 0.55 | 0.60 ± 0.89 | 0.80 ± 0.84 | 0.60 ± 0.55 | 0.80 ± 0.84 |
EOS (%) | 0.60 ± 0.89 | 1.00 ± 0.71 | 1.00 ± 0.71 | 0.80 ± 0.84 | 1.20 ± 0.84 | 0.80 ± 0.84 |
BASO (%) | 0.20 ± 0.45 | 0.20 ± 0.45 | 0.00 ± 0.00 | 0.20 ± 0.45 | 0.00 ± 0.00 | 0.20 ± 0.45 |
PT (sec) | 19.29 ± 2.95 | 16.15 ± 0.89 | 18.90 ± 1.43 | 14.50 ± 1.99* | 16.26 ± 3.13 | 16.03 ± 1.85 |
APTT (sec) | 20.90 ± 4.83 | 20.58 ± 3.25 | 18.76 ± 2.70 | 20.48 ± 1.11 | 19.99 ± 2.41 | 20.69 ± 1.80 |
Females | ||||||
RBC (106/µL) | 6.20 ± 0.95 | 6.70 ± 0.84 | 6.02 ± 0.02 | 5.91 ± 0.12 | 7.31 ± 0.58 | 7.97 ± 0.38 |
HGB (g/dL) | 11.94 ± 1.15 | 12.80 ± 1.73 | 11.68 ± 0.04 | 11.22 ± 0.22 | 13.20 ± 1.31 | 13.98 ± 0.49 |
HCT (%) | 38.02 ± 2.51 | 37.82 ± 5.50 | 45.12 ± 0.34* | 41.52 ± 0.76 | 40.22 ± 2.30 | 42.56 ± 2.47 |
MCV (fL) | 61.94 ± 5.35 | 56.38 ± 1.18* | 75.00 ± 0.38* | 70.24 ± 0.15* | 55.44 ± 2.14 | 53.36 ± 0.75 |
MCH (pg) | 19.42 ± 1.69 | 19.10 ± 0.31 | 19.42 ± 0.11 | 18.98 ± 0.25 | 18.16 ± 0.83 | 17.54 ± 0.34 |
MCHC (g/dL) | 31.38 ± 1.31 | 33.90 ± 0.51* | 25.90 ± 0.19* | 27.04 ± 0.26* | 32.76 ± 1.76 | 32.88 ± 0.86 |
PLT (103/µL) | 798.20 ± 159.93 | 850.80 ± 67.13 | 672.60 ± 3.58 | 550.00 ± 266.23 | 993.60 ± 203.13 | 888.80 ± 107.36 |
RET (%) | 1.04 ± 0.17 | 1.04 ± 0.26 | 1.08 ± 0.23 | 1.00 ± 0.14 | 1.08 ± 0.23 | 1.04 ± 0.17 |
WBC (103/µL) | 12.04 ± 1.88 | 13.40 ± 5.39 | 13.34 ± 0.11 | 15.20 ± 0.64 | 14.74 ± 3.95 | 15.32 ± 2.66 |
NEU (%) | 25.80 ± 2.39 | 26.40 ± 2.51 | 26.00 ± 1.87 | 26.00 ± 1.58 | 27.20 ± 1.92 | 26.60 ± 2.07 |
LYM (%) | 72.20 ± 2.28 | 71.80 ± 1.92 | 72.40 ± 2.07 | 72.20 ± 2.28 | 71.00 ± 1.58 | 71.60 ± 1.95 |
MONO (%) | 1.20 ± 0.84 | 0.60 ± 0.55 | 0.60 ± 0.89 | 0.80 ± 0.84 | 0.60 ± 0.55 | 0.80 ± 0.84 |
EOS (%) | 0.60 ± 0.89 | 1.00 ± 0.71 | 1.00 ± 0.71 | 0.80 ± 0.84 | 1.20 ± 0.84 | 0.80 ± 0.84 |
BASO (%) | 0.20 ± 0.45 | 0.20 ± 0.45 | 0.00 ± 0.00 | 0.20 ± 0.45 | 0.00 ± 0.00 | 0.20 ± 0.45 |
PT (sec) | 17.79 ± 3.21 | 18.95 ± 1.05 | 18.02 ± 0.48 | 14.07 ± 1.48* | 16.75 ± 1.37 | 18.36 ± 2.31 |
APTT (sec) | 16.91 ± 2.71 | 18.29 ± 0.34 | 18.50 ± 1.60 | 18.53 ± 1.29 | 18.61 ± 1.95 | 21.18 ± 1.16# |
Data is presented as Mean ± Standard Deviation (n = 5 animals per group per sex). *p < 0.05 vs. G1 (One-Way ANOVA followed by Dunnett’s post-hoc multiple comparison test), #p < 0.05 vs. G1R (Student’s t test).
RBC, red blood cell count; HGB, Hemoglobin; HCT, hematocrit; MCV, mean corpuscular volume; MCH, mean corpuscular hemoglobin; MCHC, mean corpuscular hemoglobin concentration; PLT, platelet; RET, reticulocyte; WBC, white blood cell count; NEU, neutrophils, LYM, lymphocytes; MONO, monocytes; EOS, eosinophils; BASO, basophils; PT, prothrombin time; APTT, activated partial thromboplastin time.