Table 3.
Primer sequences used in quantitative real time PCR.
| Primer name | Sequence | Product Size | Annealing temperature | Accession number |
|---|---|---|---|---|
| CAT | ||||
| For | 3-AAGGAGGAGGGAGGCAGTC-5̒ | 106 | 60.74 | AF475100 |
| Rev | 3-CAAGGCTACACGCACACAAC-5 | 60.37 | ||
| POD | ||||
| For | 3-GACCAGGACCTCTTCACCAA-5̒ | 224 | 60.09 | AB518867 |
| Rev | 3-ACGATGGTCTGCACAAAGG-5̒ | 59.69 | ||
| PPO | ||||
| For | 3-GCGACACCAGCTTCGTCTTC-5̒ | 227 | 63.42 | AY596267 |
| Rev | 3-GTACTCCTTCCGGCCCTTCTT-5 | 62.98 | ||
| GAPDH | ||||
| For | 3-CGGAAAGTTGACTGGAATGG-5̒ | 202 | 60.49 | EF592180 |
| Rev | 3-GGACCTGTTGTCACCCTGAA-5̒ | 60.97 | ||
CAT catalase, POD peroxidase, PPO polyphenol oxidase, GAPDH glyceraldeyde-3-phosphate dehydrogenase.