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. 2022 Jun 30;8:301. doi: 10.1038/s41420-022-01088-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The UMAP plot of LGE cells. b The heatmap of the LGE cells ordered by the UMAP showed in a. Each column represents expression in one cell, and each row represents expression of one gene. c The proportions of distinct clusters among total LGE cells. d UMAP plots of the expression levels of selected marker genes in the seven subpopulations. e Monocle3 revealed predicted lineage trajectories from RGC in the LGE; each point represents a cell and is colored on the basis of cluster identity. f Sp9 expression pattern in the predicted lineage trajectories, and ridge plots shows Sp9 expression distribution in the MSN Progenitor, pre-D1 MSN, D1 MSN, and D2 MSN.