Fig. 3. SP9⁺ LGE progenitors generate both D1-MSNs and D2-MSNs.

a CRE/SP9 double immunostaining in the LGE of the Sp9-Cre mice at E16.5. a’ Higher magnification images of CRE⁺/SP9⁺ cells in the LGE. b BCL11B/GFP double immunostaining in the Sp9-Cre; H2B-GFP LGE at E16.5. b’ Higher magnification images of BCL11B ⁺/GFP⁺ cells in the LGE. c ISL1⁺/GFP⁺ double immunostaining in Sp9-Cre; H2B-GFP LGE at E16.5. c’ Higher magnification images of ISL1⁺/GFP⁺ cells in the LGE. d EBF1⁺/GFP⁺ double immunostaining in Sp9-Cre; H2B-GFP LGE at E16.5. d’ Higher magnification images of EBF1⁺/GFP⁺ cells in the LGE. Scale bars: 100 µm in d for a–d, and 25 µm in d’ for a’–d’.