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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: J Am Chem Soc. 2022 May 18;144(21):9372–9379. doi: 10.1021/jacs.2c01424

Figure 2.

Figure 2.

Discovery of a candidate gnt biosynthetic gene cluster (BGC) via sequencing a guanitoxin-producing cyanobacterium. (A) Annotation of the assembled 5.24 Mbp Sphaerospermopsis torques-reginae ITEP-024 genome using antiSMASH v6.023 detected 11 candidate BGCs, while a single candidate guanitoxin BGC was identified via colocalization of relevant candidate enzyme activities. The figure was produced with Circos v0.69-8.24 The background S. torques-reginae image25 is used with permission from Dr. Vera Werner. (B) Organization of the guanitoxin BGC. The gnt locus figure was designed from NCBI accession CP080598.1 (this work) using Clinker v0.0.21.26 gnt BGC deposited under MIBiG27 accession number BGC0002148. (C) Guanitoxin biosynthetic pathway in S. torques-reginae ITEP-024. Individual Gnt enzymes are color-coded after their encoding gnt gene as per (B).