Table 2.
Phage sensitivity of A. baumannii isolated from the patient before and during phage therapy.
| Bacterial strains | Phages used for treatment | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Strain name | Date of isolation | Isolation source | Maestro | AC4 | AB- Navy1 | AB- Navy4 | AB- Navy71 | AB- Navy97 | AbTP3phi1 |
| TP1 | 02/10/2016 | 5 × 108 | 6 × 1010 | 6 × 109 | 5 × 109 | 3 × 1010 | 2 × 108 | 1 × 1010 | |
| AB-SD2 | 03/14/2016 | 6 × 108 | 3 × 1010 | 5 × 109 | 6 × 109 | 8 × 109 | 1 × 108 | ND | |
| Start of phage therapy: March 15th | |||||||||
| AB-SD4 | 03/17/2016 | Abscess-Drain 3 | R | R | Z | Z | R | Z | 3 × 109 |
| AB-2392 | 03/17/2016 | R | R | Z | Z | R | 1 × 105 | 3 × 109 | |
| Ab-2399 | 03/17/2016 | Abscess-Drain 3 | R | R | Z | Z | R | Z | 3 × 109 |
| AB-SD1 | 03/19/2016 | Abscess-Drain 5 | R | R | 1 × 105 | 1 × 106 | R | 2 × 104 | 5 × 109 |
| AB-SD3 | 03/19/2016 | Abscess-Drain 3 | R | R | 1 × 105 | Z | R | 1 × 105 | 3 × 109 |
| AB-2755 | 03/19/2016 | Abscess-Drain 1 | R | R | 1 × 104 | 1 × 104 | R | 1 × 106 | 3 × 109 |
| TP2 | 03/21/2016 | Drainage 1 | R | R | 1 × 105 | 1 × 105 | R | R | 2 × 108 |
| AB-SD5 | 03/21/2016 | Bronchial wash | R | R | R | R | R | R | 2 × 109 |
| TP3 | 03/23/2016 | Drain 1 | R | Z | Z | Z | R | Z | 1 × 109 |
| AB-0280 | 03/31/2016 | R | R | R | R | R | R | 4 × 109 | |
| AB-1485 | 05/09/2016 | Drain 3 | R | R | R | R | R | R | 1 × 107 |
| AB-3804 | 05/09/2016 | Drain 1 | R | R | R | R | R | R | 1 × 107 |
| AB-3819 | 05/09/2016 | Drain 3 | R | R | R | R | R | R | 1 × 107 |
| AB-3847 | 05/09/2016 | Drain 5 | R | R | R | R | R | R | 1 × 107 |
| AB-0214 | 05/16/2016 | Drain 5 | R | R | R | R | R | R | 2 × 107 |
| AB-0779 | 05/16/2016 | Drain 1 | R | R | R | R | R | R | 1 × 109 |
| AB-0780 | 05/16/2016 | R | R | R | R | R | R | 1 × 109 | |
Phages were titered on strain TP1 and on lawns of the isolates obtained during the course of phage therapy. At high phage concentrations (>107 PFU/ml), some phages were able to form clearing zones (denoted as Z) but no individual plaques were observed at lower dilutions. Phage resistance (no observable clearing at any phage concentration) is designated as R. All assays were performed in triplicate.