. Author manuscript; available in PMC: 2022 Jul 1.

Published in final edited form as: J Neuroendocrinol. 2021 Sep 10;34(2):e13028. doi: 10.1111/jne.13028

Table 2.

Correlation of between neurosteroid activation of tonic inhibition and seizure protection in in mouse 6-Hz model.

Neurosteroid	Tonic Currents		Seizure Protection ED₅₀ (mg/kg)^***
Neurosteroid	E_{1 μM} (pA)^*	EF_{(2-fold GABA)} (nM)^**	Seizure Protection ED₅₀ (mg/kg)^***
Brexanolone (AP)	100.6	80	4.2 (2.7–5.8)
Ganaxolone	64.0	290	1.5 (1.3–1.7)
Pregnanolone	44.4	780	7.7 (6.6–8.8)
Isopregnanolone	15.3	>10000	> 100
THDOC	66.6	410	5.0 (2.6–7.4)
Alfaxolone	40.9	990	8.8 (6.1–11.4)
ORG-20599	86.4	120	18.6 (16.6–20.6)
Androstanediol	33.2	1710	44.0 (30.2–58.8)

E_{1 μM} values represent the mean normalized tonic current responses of drug at 1 μM concentration co-applied with 1 μM GABA. GABA 1 μM tonic current: 0.66 ± 0.22 pA/pF, 19.6 pA.

^**

EF values represent the effective functional concentration of drug (nM) required to double or triple the 1 μM GABA response.

^***

ED₅₀ values represent the dose in milligrams per kilogram that protected 50% of animals in the 6-Hz seizure stimulation test. 95% confidence intervals are listed in parenthesis, according to a normal distribution.