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. 2022 Jun 17;12:889711. doi: 10.3389/fcimb.2022.889711

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Copyright © 2022 Chahal, Quintana-Hayashi, Gaytán, Benktander, Padra, King and Linden

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Insoluble salivary mucins and their binding to lectins and S. oralis. Fractions from CsCl density gradient centrifugation of the insoluble saliva fractions (solubilized by reduction) were collected from the bottom of the tube, diluted 1:20 and analyzed for: (A, B) carbohydrate, MUC5B, MUC7 and SAG. (C, D) Fractions were probed with the lectins SNA-I for α2,6-linked sialic acid, MAA for α2,3-linked sialic acid and ECL for Galβ1-related oligosaccharides. To visualize the MAA and ECL signals in the graphs, their signals were multiplied by 10. (E, F) Fractions were assessed for S. oralis IE12 WT, ATCC 10557 WT and F0392 WT binding.