FIGURE 7.

The effects of ODQ after sGC activation by (A) the sGC stimulator BAY 41-2272 (10 μΜ) alone or (B) in combination with the NO donor SNP (100 μΜ), in A7r5 rat aortic smooth muscle cells. The heme oxidant ODQ (10 μΜ) or its vehicle (DMSO) was added either 20 min before or 2 min after the sGC agonists. 15 min after the exposure to the agonists, cell extracts were taken, analyzed for cGMP content and normalized for total protein. Data are expressed as means ± STDEV of n = 4 determinations, obtained in two independent experiments. p values were determined by 2-tailed Student’s t-test. (A). The effect of BAY 41-2272 in the absence of ODQ was taken as 1.0. ***: p < 0.001 relative to BAY 41-2272 treatment in the absence of ODQ. (B). The effect of BAY 41-2272 + SNP in the absence of ODQ was taken as 1.0. ***: p < 0.001 relative to BAY 41-2272 + SNP treatment in the absence of ODQ.