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. 2022 Jun 17;13:884434. doi: 10.3389/fimmu.2022.884434

Figure 5.

Figure 5

Representation of comparative diversity analyses of the VH domains of Ighμ bighead catfish, North African catfish and their hybrid catfish. General structures of the VH domain correspond to the VH, DH, and JH segments that consist of the FR1, CDR1, FR2, CDR2, FR3, CDR3 and FR4 regions found in all vertebrates (A). Comparative classification of the VH, DH, and JH gene segments in bighead catfish, North African catfish and their hybrid catfish (B). Comparison of VH gene family members among reported vertebrates (C). Degree of sequence variability of the VH repertoire in bighead catfish, North African catfish and their hybrid catfish, according to the Shannon (1948) and Kabat and Wu (1971) methods (D). Comparison of amino acid length in CDR1, CDR2, and CDR3 of bighead catfish, North African catfish and their hybrid catfish (E), x̅: average nucleotide length, S2: variance distribution. Percent frequency of amino acid variability and composition of CDR1 CDR2 and CDR3 regions of bighead catfish, North African catfish and their hybrid catfish (F). The NCBI accession numbers of VH nucleotide sequences are MN934442- MN934541, MN934542- MN934641 and MN934642- MN934741 for bighead catfish, North African catfish and their hybrid catfish, respectively.1Yasuike et al., 2010 (44); 2Danilova et al., 2005 (13); 3Yang et al., 2003 (45); 4Brown et al., 2006 (46); 5Phuyindee et al., 2015 (47); 6Andersson and Matsunaga, 1998 (48); 7Stenvik et al., 2000 (49); 8Wilson et al., 1991 (31); 9Peixoto and Brenner, 2000 (50); 10Coscia and Oreste, 2003 (22); 11Rumfelt et al., 2004 (51); 12Lundqvist et al., 1998 (52); 13Haire et al., 1990 (53);14Ota and Nei, 1995 (54); 15Mainville et al., 1996 (55); 16Mage et al., 1984 (56); 17Sun et al., 1994 (57) and 18Matsuda et al., 1998 (58).