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. 2022 Jun 17;13:884434. doi: 10.3389/fimmu.2022.884434

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Copyright © 2022 Bunnoy, Na-Nakorn and Srisapoome

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representation of the overall relative expression and distribution of Ighμ in bighead catfish, North African catfish and their hybrid catfish in sixteen tissues (A). Comparison of the relative expression of Ighμ in sixteen different tissues (B). Expression responses of Ighμ in immune-related organs, including the spleen, head kidney and PBLs, after intraperitoneal injection of a virulent pathogen, Aeromonas hydrophila, of bighead catfish, North African catfish and their hybrid catfish (C). The relative expression levels of Ighμ genes were calculated using β-actin as a reference gene. All quantitative data are presented as the mean ± standard deviation (SD). The levels of statistical significance are indicated by *(P<0.05), **(P<0.01) or ***(P<0.001). The brain RT was used as the calibrator. All experiments are done in triplicate (n = 3). ns, not significant.