Table 2.
Peptides from the keratins and KAPs that contained consistently labeled cysteines and were detected in one or more of the 5 repeats of a treatment condition
| Sequence | Significance/conditions |
Protein ID | Position | Region | Fig. 8 label | |||
|---|---|---|---|---|---|---|---|---|
| Wet |
Dry (55% RH) |
|||||||
| 1a hold | 1b break | 2a hold | 2b break | |||||
| Consistently labeled cysteines irrespective of water and stretch conditions | ||||||||
| A489SSFSCGSSR | 5∗∗∗ | 4∗∗ | 5∗∗∗ | 5∗∗∗ | K85 | T-65 | tail domain | A |
| C31CISAAPYR | 5∗∗∗ | 5∗∗∗ | 4∗∗ | 3∗ | K85 | H-31, H-32 | head domain | A |
| Consistently labeled cysteines only when wet (hydrogen bonds disrupted) and both stretch conditions | ||||||||
| T12GPATTICSSDKFCR | 5∗∗∗ | 4∗∗ | 2 | 2 | KAP3.2 | 19, 25 | close to N-terminus | B |
| S98SSCSSLSSGSR | 4∗∗ | 3∗ | 2 | KAP13.1 var1 | 102 | in the middle (protein is 166 residues long) | B | |
| G25CGCGSFR | 5∗∗∗ | 3∗ | 1 | KAP19.5 | 26, 28 | in the middle (protein is 72 residues long) | B | |
| T380ITPCISSPCAPAAPCTPCVPR | 5∗∗∗ | 5∗∗∗ | 1 | 1 | K31 | T-22, T-27, T-33, T-36 | close to C-terminus | B |
| S394RFGPCNTSGC | 5∗∗∗ | 3∗ | 2 | 1 | K33b | T-37, T-42 | C-terminal peptide | B |
| S53VCGGFR | 4∗∗ | 3∗ | 2 | 2 | K83 | H-55 | head domain | B |
| V492GGSILGCKK | 5∗∗∗ | 5∗∗∗ | 2 | K86-like | T-70 | C-terminus but one, terminal cysteine | B | |
| Consistently labeled cysteines when wet and stretched and held only | ||||||||
| L125GYGICGFPVLSSGSGFCQPTFFASR | 4∗∗ | KAP13.1 var1 | 130 | close to the C-terminus (protein is 166 residues long) | C | |||
| T124FRTSPCC | 3∗ | 2 | KAP2.3 var3 | 130, 131 | C-terminal peptide | C | ||
| T12GPATTICSSDKF | 3∗ | 1 | KAP3.2 | 19 | close to N-terminus | C | ||
| Consistently labeled cysteines only in dry condition (reinforcing hydrogen bonds present) and both stretch conditions | ||||||||
| Y306SCQLSQVQSLIVNVESQLAEIR | 3∗ | 3∗ | K31, K33b | 2-90 | coil 2B | D | ||
| Consistently labeled cysteines when dry and stretched and held only | ||||||||
| V179ESLKEELICLK | 3∗ | 1 | K31 var1, K33B | 1B-87 | coil 1B (seq. number for K31) | E | ||
| V179ESLKEELLCLK | 3∗ | 1 | K34 | 1B-87 | coil 1B | E | ||
| Y306SCQLNQVQSLISNVESQLAEIR | 3∗ | 2 | K31, K33b | 2-90 | coil 2B | E | ||
| Consistently labeled cysteines only in stretch and hold condition but both wet and dry | ||||||||
| S121ISTVCQPVGGVSTICQPTCGVSR | 5∗∗∗ | 2 | 3∗ | KAP11.1 | 126, 136, 140 | close to the C-terminus (residue 158) | F | |
| Consistently labeled cysteines only when fiber stretched to break and dry | ||||||||
| A214DLEAQVESLKEELLCLK | 1 | 3∗ | K35 | 1B-87 | coil 1B | G | ||
| Consistently labeled cysteines in wet (both stretch conditions) and in dry stretched and held but not when dry stretched to break | ||||||||
| C395GPCNTFVH | 3∗ | 3∗ | 3∗ | 2 | K33a | T-33, T-36 | tail domain | H |
Statistical likelihood is marked as: ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.