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. 2022 Jun 17;10:935403. doi: 10.3389/fcell.2022.935403

FIGURE 2.

FIGURE 2

(A) Metformin concentration in trophoblasts treated in vitro with: (0.01mM and 0.1 mM) and placental tissue exposed in vivo. There was a non-significant difference between tissue metformin concentrations and metformin concentrations in 0.01 mM-treated cells (p = 0.08). There was no significant difference between tissue metformin concentrations and metformin concentrations in 0.1 mM-treated cells (p = 0.894). Metformin concentration is plotted on a log10 scale. Individual data points each represent a single trophoblast sample with points more than 1.5x IQR highlighted with larger dots. Light grey boxes: trophoblasts treated with 0.01 mM metformin; Dark grey boxes: 0.1 mM metformin; white boxes: Placental tissue from patients treated with metformin in vivo. (B) mRNA level of potential metformin transporters in trophoblasts (n = 30 samples). Genes assayed: OCT-1 (SLC22A1), OCT-2 (SLC22A2), OCT-3 (SLC22A3), MATE-1 (SLC47A1), MATE-2 (SLC47A2), SERT (SLC6A4), ThTR2 (SLC19A3), NET (SLC6A2) and ENT-4 (SLC29A4). All copy numbers expressed relative to genomic DNA standard curve run on every plate. (C) Relative protein levels of SERT (SLC6A4), ThTR2 (SLC19A3), NET (SLC6A2) [All proteins were present at densities significantly above background (p < 0.05)].