TABLE 1.

Radioligand binding affinities for human S1P₁ and S1P₅.

Compound	Human S1P1 Ki (nM)	Human S1P5 Ki (nM)
Ozanimod	0.5 ± 0.03	1.93 ± 0.07
S1P	2.33 ± 0.17	2.69 ± 0.31
FTY720-p	0.1 ± 0.01	0.26 ± 0.03
Amiselimod-p	0.13 ± 0.02	0.25 ± 0.04
Ponesimod	9.37 ± 1.31	17.43 ± 4.32
Siponimod	0.74 ± 0.09	0.43 ± 0.08
KRP-203-p	0.22 ± 0.02	0.24 ± 0.04
Etrasimod	6.73 ± 0.62	16.3 ± 0.71

[³H]-ozanimod was competitively displaced with increasing concentration of test compound using Chinese hamster ovary cell membranes stable expressing recombinant human S1P₁ or human S1P₅. Data shown are the mean ± standard error of the mean of the binding affinities calculated for 3–5 replicate experiments run in duplicate wells.

S1P, sphingosine 1-phosphate; S1P₁, sphingosine 1-phosphate receptor subtype 1; S1P₅, sphingosine 1-phosphate receptor subtype 5.