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. 2022 Jun 17;13:903258. doi: 10.3389/fmicb.2022.903258

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Copyright © 2022 Liu, Zhang, Deng, Wang and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Thiamine-related genes contribute to PstDC3000 virulence. (A) Tomato leaves inoculated by infiltration with PstDC3000 wild type and the thiD/E, thiS/G, thiD/E/S/G, apbE mutants, and complementation strains. Phosphate-buffered saline (PBS) was used as a negative control. (B) PstDC3000 wild type, the thiD/E, thiS/G, thiD/E/S/G mutants, and their corresponding complementation strains with exogenous 100 μg/ml thiamine inoculated into tomato leaves. Pictures were taken at 7 days postinoculation (dpi). “+thi:” added exogenous 100 μg/ml thiamine. Each strain was inoculated onto 10 tomato leaves with a needleless syringe in each experiment. Experiments were repeated three times.