Figure S2.

Cvm1 is not redundant with ERMES or the Vps39-mediated vCLAMP. Related to Figs. 2 and 3. (A) Analysis of the levels in whole-cell lysate of the marker proteins used to assess copurification of mitochondria with vacuoles in Fig. 2, E and F, and in Fig. 3, C and D. Pgk1 is used as a loading control. The levels of the marker proteins are not significantly affected by the different genotypes used. (B) Analysis of the genetic interaction of Cvm1 with the ERMES complex. Cells carrying the mmm1-1 temperature-sensitive allele, plus the indicated deletions or genomic modifications, were spotted as serial dilutions in YPD plates and grown at 23 or 37°C. (C) Analysis of the genetic interaction of Cvm1 with the Vps39^12xM vCLAMP-impaired allele. Cells of the indicated genotypes were spotted as serial dilutions in YPD plates with or without 3 mM ZnCl₂ and grown at 30°C. (D) Analysis of the genetic interaction of Cvm1 with the Vps39^12xM vCLAMP-impaired allele. Cells of the indicated genotypes were diluted to OD₆₀₀ = 0.1 in 96-well plates in YPD or YPD⁺ 3 mM ZnCl₂ at 30°C. Absorbance at 600 nm was recorded using a plate reader during ∼16 h, and duplication times were calculated from the exponential phase of the growth curve. ***, P < 0.001. Source data are available for this figure: SourceData FS2.