Skip to main content
. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Angew Chem Int Ed Engl. 2021 May 14;60(25):14163–14170. doi: 10.1002/anie.202102453

Figure 4.

Figure 4.

Biochemical characterization of Bnd4, structural elucidation of 2, and biosynthesis of 1. (A) Bnd4 and TSAa catalyze the cyclization of GGPP into 2. Enzyme reactions, including a no enzyme control (GGPP), were analyzed by HPLC by monitoring at 210 nm. (B) GCMS spectrum of pure 2. (C) Structure of 2 and key 2D NMR correlations supporting its structural assignment. (D) Biosynthetic gene cluster (bnd) and proposed biosynthetic pathway for 2. The formation of 2, a novel 6,10-bicyclic scaffold, by a unique TS, precedes oxidative tailoring affording 1.