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In-vitro tri-lineage differentiation assay. DPSC and SHED were induced to differentiate into; (4A & 4D) osteocytes like cells. Calcium deposits were detected by Alizarin Red S staining. (4B & 4E) Adipocytes like cells. Oil droplets produced by these cells were detected by Oil Red O stain. (4C & 4F) Chondrocytes like cells. Sulphated glycosaminoglycans (GAGs) produced by these cells were detected by staining with toluidine blue. Scale bar = 100μm.
