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. 2022 Jun 22;2022:2253436. doi: 10.1155/2022/2253436

Figure 2.

Figure 2

ART promotes to polarize MDSCs from M2-like protumoral phenotype towards M1-like antitumoral one. (a–g) Purified CD11b+Gr-1+ MDSCs from the tumors of B16F10 melanoma cell-bearing C57BL/6 mice treated with DMSO for 13 days or ART (50 mg/kg) for 18 days (both groups had the similar tumor volume ~1000 mm3). (a and b) Performed RNA sequencing (RNA-seq) and gene set enrichment analysis (GSEA) and (c) the expressions of M1 and M2 signature genes were detected by qPCR in tumor MDSCs. (d) The cytokines IL-6, IL-10, TNF-α, and TGF-β were detected by ELISA. (e) The expressions of p-STAT1 (Tyr701), STAT1, iNOS, ARG1, and p47phox protein were detected by western blot. (f and g) Detected arginase activity by biochemical assays and nitric oxide content by DAF-FM DA fluorescence using flow cytometry analysis. Data are means ± SEM and are from a representative experiment of three (f and g) or from two (c) independent experiments. Unpaired Student's t test for (c) and (d) and (f) and (g). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001. ns: not significant.