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. 2022 Jun 22;2022:2253436. doi: 10.1155/2022/2253436

Figure 3.

Figure 3

ART controls the functional polarization of MDSCs through PI3K/AKT, mTOR, and MAPK pathways. (a and c) Purified CD11b+Gr-1+ MDSCs from the tumors of B16F10-bearing C57BL/6 mice for 13 days and then treated MDSCs with DMSO or ART (100 μM) in vitro for different time points. (a) Western blot was used to detect MAPK signal pathway-related proteins p-ERK (Thr202/Tyr204), ERK, p-p38 MAPK (Thr180/Tyr182), and p38 MAPK. (b) Gray value statistics. (c) PI3K/AKT and mTOR signaling pathway-related proteins p-AKT (ser473), AKT, p-p70 S6K (Thr389), and p70 S6K were detected by western blot. (d) Gray value statistics. Data are means ± SEM and are from a representative experiment of three (a and c). Unpaired Student's t test for (b) and (d). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001. ns: not significant.