Figure 3.

Functional analysis of beige adipose tissue formation in direct adipogenic culture of lean and diabetic microvascular fragments. Microvascular fragments (MVF) from subcutaneous fat of lean (L) and diabetic (Db) rats were directly exposed to white (WAM) or beige (BAM) adipogenic media for 14 days. At the end of the 14 days (a) Insulin stimulated glucose uptake (ISGU) ± insulin, to stimulate glucose uptake and (b) lipolysis ± isoproterenol, to stimulate lipolysis, was measured. (c–g) Oxygen Consumption Rate (OCR) trace was determined using a Seahorse XF96 Analyzer among the different groups, basal respiration, proton leak, maximal respiration, and spare capacity were calculated. Results are reported as mean ± standard error of two experimental replicates (n = 4 per experiment). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.