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. 2022 Jun 30;20:296. doi: 10.1186/s12967-022-03476-7

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — High CHAF1A exerts tumor-promoting biological effects in DLBCL. A The RT-qPCR analysis indicated the down-regulated CHAF1A mRNA expressions shCHAF1A DLBCL cell lines (DB, U2932 and FARAGE) and shCtrl cells. B The RT-qPCR analysis indicated the elevated CHAF1A expressions in EV and OE OCI-Ly7 cells. C MTT assay showed suppressed cell viability when CHAF1A was knocked down by shCHAF1A in three independent DLBCL cell lines as compared to control (shCtrl) cells. D In contrast, CHAF1A overexpression could cellular proliferative activity of OCI-Ly7. E The colony generation of DLBCL cells by colony formation assays showed CHAF1A KD could attenuate colony formation ability, but CHAF1A OE could rescue the suppressed capacity. F Besides, CHAF1A KD could also attenuate migration ability of cells by Transwell assay, but CHAF1A OE could also rescue the impaired migration capacity. G Animal assays on nude mice with OCI-Ly7 cells showed enhanced tumor growth rate in the CHAF1A OE group compared with the control group (EV). Representative tumor graphs were shown on the left, while H&E and IHC staining of Ki-67 were exhibited on the right. Scale bar = 50 μm H Record of tumor volumes at the indicated timepoints and generation of tumor growth curves in two mice groups. I After the animal sacrifice, tumor weight of mice derived from the two groups were compared. Experiments were performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001