Fig. 3.

Loss of tau does not affect end-stage neuropathology in TgA53T mice. Spinal cord sections from end-stage nTg, mTau^−/−, TgA53T and TgA53T/mTau^−/− mice were stained for pS129 αS (a–e), Iba1 (microglia) (f–j), and GFAP (astrocytes) (k–o). Shown are representative images and corresponding quantitative analysis of % area covered by immunoreactivity in the lumbar region. In both TgA53T and TgA53T/mTau^−/− mice the indices of neuropathology were significantly increased to a similar extent. One-way ANOVA with Tukey’s post-hoc analysis. pS129 αS: F_(3,40) = 30.64; P < 0.0001. Iba1: F_(3,40) = 54.81; P < 0.0001. GFAP: F_(3,20) = 88.16; P < 0.0001. n = 6–15 sections from 3 to 5 animals/genotype; scale bars = 100 μm; error bars represent mean ± SEM