Table 3.

Comparison of detected concentrations between preparation with a 3 K filter and acid precipitation. Three repeats for each method were performed.

Amino Acid	3 K Filter Avg. μM	Acid Precipitation Avg. μM	Ratio Acid Precip. to 3 K Filter
Glutamic Acid	87.05 ± 0.52	70.97 ± 3.04	1.23
Asparagine	54.13 ± 0.15	46.93 ± 1.04	1.15
Serine	97.05 ± 0.8	84.99 ± 4.32	1.14
Glutamine	601.06 ± 1.35	554.32 ± 12.83	1.08
Histidine	54.56 ± 0.15	48.11 ± 2.86	1.13
Glycine	233.06 ± 0.45	237.97 ± 8.58	0.98
Threonine	85.23 ± 0.75	76.69 ± 2.02	1.11
Citrulline	12.94 ± 0.34	11.68 ± 0.75	1.11
Arginine	74.89 ± 0.18	72.47 ± 1.7	1.03
Alanine	408.51 ± 40.97	334.81 ± 20.05	1.22
Tyrosine	59.23 ± 0.35	54.66 ± 1.73	1.08
Valine	218.43 ± 0.25	199.63 ± 4.49	1.09
Methionine	17.52 ± 0.14	16.38 ± 0.76	1.07
Cystine	144.29 ± 0.65	130.49 ± 3.35	1.11
Beta alanine	40.89 ± 0.36	37 ± 3.35	1.11
Tryptophan	6.34 ± 0.21	44.43 ± 1.24	0.14
Isoleucine	44.14 ± 0.49	44.7 ± 1.42	0.99
Phenylalanine	68.42 ± 0.38	62.53 ± 1.29	1.09
Leucine	123.74 ± 0.3	114.9 ± 2.97	1.08
ornithine	78.88 ± 0.72	72.85 ± 3.76	1.08
Lysine	173.1 ± 0.21	165.41 ± 5.92	1.05
Ammonium	11.98 ± 0.27	16.91 ± 3.03	0.71